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Registros recuperados: 225
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Infection with the protozoan parasite Bonamia ostreae modifies in vitro haemocyte activities of flat oyster Ostrea edulis ArchiMer
Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan.
Bonamia ostreae is an intracellular protozoan parasite, infecting haemocytes of the European flat oyster Ostrea edulis. Oyster defence mechanisms mainly rely on haemocytes. In the present study in vitro interactions between parasites and flat oyster haemocytes were investigated using flow cytometry and light microscopy. Haemocyte parameters including: non specific esterase activity, reactive oxygen species (ROS) production and phagocytosis were monitored using flow cytometry after 2 hour cell incubation with live and dead B. ostreae. Two ratios of parasites per haemocyte were tested (5:1 and 10:1), haemocytes alone were used as controls and the experiment was carried out three times. Flow cytometry revealed a decrease of non specific esterase activities...
Tipo: Text Palavras-chave: Reactive oxygen species (ROS); Esterase; Flow cytometry; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6271.pdf
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Influence de la température sur la filtration et la consommation d'oxygène de l'huître plate Ostrea edulis: détermination des coefficients allométriques ArchiMer
Haure, Joel; Penisson, Christian; Bougrier, Serge; Baud, Jean-pierre.
La filtration et la consommation d'oxygène d'Ostrea edulis ont été mesurées à différentes températures (10°-30°C) sur des individus de 5 à 120g de poids total (0,1 à 2,7g de poids sec) et hors période de gamétogénèse. Pour chaque température, la relation allométrique entre la réponse physiologique et le poids sec de l'animal (W) a été estimée selon la relation: y=aWb. Un modèle statistique est proposé pour expliquer la filtration ou la consommation d'oxygène (y) en fonction de la température (T) et du poids sec de l'animal (W): y=[a+(b.cT)]. Wd.d est égal à 0,62 pour la filtration et à 0,74 pour la consommation d'oxygène. Les deux fonctions physiologiques augmentent avec l'élévation de la température et atteignent leur maximum à 30°C ( Filtration:...
Tipo: Text Palavras-chave: Filtration; Consommation oxygène; Poids sec; Température; Ostrea edulis.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00048/15886/13308.pdf
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Influence of diet assemblage on Ostrea edulis broodstock conditioning and subsequent larval development ArchiMer
Gonzalez, Ricardo; Mingant, Christian; Petton, Bruno; Robert, Rene.
In contrast with the Japanese oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum, Ostrea edulis seed production in the hatchery has been reported to be erratic, with sudden and unexplained larval and post-metamorphosis mortalities. Fecundity and initial larval quality have been related to broodstock conditioning, but effects on larval development and metamorphosis remain poorly understood. In addition, molluscan larval mortalities have been often associated with bacterial contamination and flow-through techniques may help to overcome this problem. Both aspects have been considered in the present work. O. edulis broodstock were conditioned at 19 °C and fed three different microalgal diets. Two were single-species diets: Rhodomonas salina...
Tipo: Text Palavras-chave: Ostrea edulis; Feeding; Broodstock conditioning; Larval development; Flow-through.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00090/20093/17732.pdf
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Influence of feeding regime and temperature on development and settlement of oyster Ostrea edulis (Linnaeus, 1758) larvae ArchiMer
Robert, Rene; Vignier, Julien; Petton, Bruno.
Under controlled conditions of food density and temperature, larval performances (ingestion, growth, survival and settlement success) of the flat oyster, Ostrea edulis, were investigated using a flow-through rearing system. In the first experiment, oyster larvae were reared at five different phytoplankton densities (70, 500, 1500, 2500 and 3500 μm3 μL−1: ≈1, 8, 25, 42 and 58 cells μL−1 equivalent TCg), and in the second, larvae were grown at four different temperatures (15, 20, 25 and 30°C). Overall, larvae survived a wide range of food density and temperature, with high survival recorded at the end of the experiments. Microalgae concentration and temperature both impacted significantly larval development and settlement success. A mixed diet of Chaetoceros...
Tipo: Text Palavras-chave: Ostrea edulis; Larvae; Temperature; Feeding regime; Flow-through; DEB parameters.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00377/48817/49295.pdf
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Integration of shellfish farming activies in three French coastal environments : Mont Saint-Michel Bay, Gulf of Morbihan, and Marennes-Oleron Bay ArchiMer
Mazurie, Joseph; Le Mao, Patrick; Camus, Patrick; Bacher, Cedric; Goulletquer, Philippe.
Integration of shellfish farming along the French shoreline (around 5500 km) has been progressive, as marine bivalve culture is a traditional activity in our country. The history of mussel culture relates that the "bouchot" technique (intertidal culture on wooden poles, producing annually around 60,000 tons) was developed as early as the XIIIth century, by a refugee attempting to capture birds by sticking branches of brushwood along the shore. The European flat oyster, Ostrea edulis, already appreciated at the Roman period, was first cultivated in France after Victor Coste introduced the technique of spat collection at the end of the XIXth century. The Pacific cupped oyster, genus Crassostrea, now the most-cultivated in France (around 120,000 tons marketed...
Tipo: Text Palavras-chave: Trophic capacity; Trophic resource; Nature conservation; Coastal zone management; Biodiversity; Mytilus edulis; Mussels; Integration; Ostrea edulis; Crassostrea gigas; Oysters.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3400.pdf
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Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? ArchiMer
Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle.
Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to...
Tipo: Text Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf
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La bonamiose: caractérisation du parasite Bonamia ostreae et étude des ses interactions avec l'hôte, l'huître plate Ostrea edulis ArchiMer
Besnard-cochennec, Nathalie.
La bonamiose, maladie due au protozoaire Bonamia ostreae a été détectée en 1979 pour la première fois en Bretagne au cours d'épisodes de mortalité. Depuis, cette maladie s'est propagée à tous les centres ostréicoles français, puis européens. Cette étude a pour objectif de décrire les compartiments du modèle B. ostreae/hémocyte de l'huître plates, Ostrea edulis et d'analyser leurs interactions in vivo et in vitro. La caractérisation enzymatique, antigénique et moléculaire du parasite a été réalisée. Ceci a permis de disposer d'une palette de réactifs pour la comparaison de ce parasite avec d'autres protozoaires. Ainsi, un parasite de l'huître creuse chinoise Crassostrea rivularis a té décrit, puis rapproché du genre Bonamia. La morphologie et l'équipement...
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Huître creuse chinois; Crassostrea rivularis; Bonamia ostreae; Enzymes; Résistance; Mécanisme de défense.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00211/32198/30635.pdf
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La bonamiose de l' huître plate Ostrea edulis. Bilan des connaissances actuelles, analyse des données collectées en France de 1989 à 2004, mesures de lutte et perspectives ArchiMer
Blanchet, Estelle.
Bonamiosis, favoured by change of temperature and stress, causes mortalities in the adult flat oyster Ostrea edulis in the majority of European oyster farmers. The life cycle of this Haplosporidia protozoan Bonamia ostreae is unknown, it is not dttectable during the five mon th latent period. Because of the Jack of sensibility of reference techniques, histology and tissue smear, further use of PCR assays or HCM-CC, in particular, is recommended. Analysis of data collected in France from 1989 to 2004 shows the absence of simple prediction methods and an apparent cyclicity of the disease. Transfer restriction and control of boat hulls can protect uninfected areas. Reducing stress and density, commercialising earlier can limit losses in infected areas. The...
Tipo: Text Palavras-chave: Bivalve; Bonamia ostreae; Epidémiologie; Haplosporidia; Huître plate; Lutte; Ostrea edulis; Protozoaire; Surveillance; Bonamia ostreae; Control; Epidemiology; Flat oyster; Haplosporidia; Ostrea edulis; Protozoan; Survey.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/00451/56235/57795.pdf
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La Bonamiose : études des mécanismes cellulaires de défense de l'huître plate, Ostrea edulis, vis à vis du parasite Bonamia ostreae ArchiMer
Cochennec, Nathalie; Renault, Tristan; Chollet, Bruno.
Bonamiosis—a disease caused by a protozoan parasite, Bonamia ostreae—was detected for the first time in Brittany in 1979 in conjunction with a massive mortality event. Since then, the disease has spread to all French oyster farming areas, and then throughout Europe.
Tipo: Text Palavras-chave: Pathologie; Ostrea edulis; Bonamia ostreae.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3241.pdf
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La bonamiose un fléau de l'huître plate.... ArchiMer
Morga, Benjamin.
The history of French oyster culture shows very well how fragile this farming is to stock overexploitation and diseases. The production of flat oyster, Ostrea edulis, fell dramatically after the appearance of two parasitic diseases: arteiliosis and bonamiosis. The curative means against bonamiosis (Bonamia ostreae) are restricted and imply a good knowledge of the parasite's life cycle and of its interaction with its host. Studying the expression of the parasite's and the host's genes during the infection seems essential in providing new adapted measures in order to maintain, if not relaunch the flat oyster culture. In this context, this thesis aims to understand the flat oyster-parasite interactions, and especially, the molecular basis of the resistance to...
Tipo: Text Palavras-chave: Biologie moléculaire; Gene; Parasite; Huîtres; Ostrea edulis.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3369.pdf
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La fixation en claires des larves de l'huître plate Ostrea edulis ArchiMer
Trochon, Pierre.
La reproduction des huîtres plates dans les claires de la région de La Tremblade a été étudiée à partir de l'année 1953. Les premières observations permirent de constater dans ces plans d'eau la présence de larves en grand nombre au début de l'été ; mais la quantité des larves parvenues au stade de fixation demeurait toujours très faible. Néanmoins, sur des collecteurs immergés aux moments les plus favorables, de très faibles fixations furent obtenues. Il était donc démontré qu'il était possible de récolter des huîtres plates dans ces bassins.
Tipo: Text Palavras-chave: Reproduction; Huître plate; Ostrea edulis; Claires ostréicole; Marennes Oléron.
Ano: 1956 URL: http://archimer.ifremer.fr/doc/00005/11578/8203.pdf
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La maladie de la glande digestive d'Ostrea edulis Linne ArchiMer
Grizel, Henri; Tige, Gilbert.
The first microscope observations of the digestive gland of Ostrea edulis were conducted by Comps (1970) and Herbach (1971), but the first effects had already been observed in the autumn of 1967. During summer 1968, an abnormal mortality occurred in the upstream part of the Aber Wrach, then spread downstream and into the Aber Benoît. Similar mortalities were also observed in the area of La Tremblade and in the Arcachon Bay. Since then, a permanent monitoring is conducted in the different Breton oyster culture centres and the Arcachon area.
Tipo: Text Palavras-chave: Glande digestive; Maladie; Ostrea edulis; Huître; Pathologie.
Ano: 1973 URL: http://archimer.ifremer.fr/doc/1973/acte-5929.PDF
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La variabilité génétique chez l'huître plate Ostrea edulis (L. 1758) : inférence du nombre de géniteurs instantané d'une population ArchiMer
Da Silva, Sara Maria Mira.
Les études de génétique des populations des bivalves marins ont fréquemment mit en évidence une relation hétérozygotie / valeur sélective, et un fort déficit en hétérozygotes. Parmi les hypothèses explicatives de ces deux phénomènes, la création d'individus consanguins dans les populations est particulièrement intéressante à tester. Le but de ce travail était de vérifier s'il pouvait y avoir un nombre faible de géniteurs à la base d'un événement de reproduction donné chez l'huître plate Ostrea edulis. Dans cette étude, nous montrons qu'une cohorte de captage collectée sur un intervalle de temps très court (15 jours), présente une diversité génétique fortement réduite ainsi qu'une modification des fréquences alléliques par rapport aux populations d'adultes...
Tipo: Text Palavras-chave: Génétique population; Huître plate; Ostrea edulis; Diversité génétique; Variabilité génétique.
Ano: 2001
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L'amélioration des performances de l'huître plate européenne Ostrea edulis par la sélection génétique ArchiMer
Bedier, Edouard; Baud, Jean-pierre; Cochennec, Nathalie; Cornette, Florence; Gerard, Andre; Goyard, Emmanuel; Joly, Jean-pierre; Kopp, Joel; Langlade, Aime; Le Coguic, Yvette; Mazurie, Joseph; Nourry, Max.
The European flat oyster Ostrea edulis has been considered as the traditional French oyster for a very long time and has now nearly disappeared from our farms, as a consequence of the two successive epizootic of the Marteilia refringens parasite and Bonamia ostreae. The actual quasi monoculture of Crassostrea gigas has made necessary the search for a diversification in oyster culture, including the search for a new start of the indigenous flat oyster culture. The Ifremer has been conducting parallel works since 1985 in order to better understand the biology of the parasite as well as to try and select different lines of flat oyster resistant to Bonamia ostreae.
Tipo: Text Palavras-chave: Bonamia ostrea; Huître plate; Sélection Génétique; Ostrea edulis.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3274.pdf
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Larval development and fatty acid composition of Ostrea edulis (L.) fed four different single diets from conditioning to pre-settlement ArchiMer
Gonzalez-araya, Ricardo; Robert, Rene.
Survival, growth and fatty acid composition of Ostrea edulis larvae (L.) fed four different single species, microalgal diets, Tisochrysis lutea (T), Chaetoceros neogracile (Cg), Skeletonema marinoi (Sm) or Tetraselmis suecica (Ts) from broodstock to pre-settlement, were studied. Lower larval growth (5.5m to 6.5m/d) was recorded in progeny continuously fed single S.marinoi or T.suecica, whereas good growth was achieved with single T.lutea (7.8m/d). Larvae, originated from broodstock receiving Sm or Ts, exhibited growth compensation when fed a bispecific balanced diet (TCg). This did not occur when broodstock and larvae were fed Cg or T, for which single or mixed diets led to similar larval growth. Furthermore, survival was high (>90%) regardless of...
Tipo: Text Palavras-chave: Broodstock conditioning; Diet; Larval development; Ostrea edulis.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00438/54986/56483.pdf
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Les huîtres des côtes françaises Ostrea edulis et Gryphaea angulata ArchiMer
Lambert, Louis.
Les bancs d'huîtres plates Ostrea edulis formaient jadis un cordon ininterrompu sur toutes les côtes de France. Quelques chiffres donneront une idée des quantités d'huîtres pêchées jadis sur les côtes françaises. A la fin du XVIII ème siècle et au début du XIV ème, les pêcheurs enlevaient des seuls bancs de Cancale et Granville au minimum cent millions d'huîtres par ans; leurs bateaux pêchaient du 1er Septembre au 1er Avril sans discontinuer. La destruction du naissain et des petites huîtres par les dragues très lourdes que l'on employait alors était plus grand encore. L'exportation, de 1772 à 1775, atteignit le chiffre de 293 millions. En 1775, notamment, 234 bateaux normands et 104 anglias emportaient de la baie de Cancale 95 millions d'huîtres.
Tipo: Text Palavras-chave: Huître plate; Huître portugaise; Ostrea edulis; Gryphaea angulata; Ostréiculture; Récolte naissain; Technique élevage; Ennemis; Maladies; France.
Ano: 1949 URL: http://archimer.ifremer.fr/doc/00004/11491/8055.pdf
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L'évolution de la maladie de la glande digestive de l'huître plate dans le bassin d'Arcachon et ses conséquences actuelles ArchiMer
Tige, Gilbert; His, Edouard; Rabouin, Marie-agnès.
Nous avions montré qu'en 1975 la maladie de la glande digestive de l'huître plate était responsable d'une mauvaise qualité et de mortalités importantes d'huîtres plates du bassin d'Arcachon (His & coll., 1976). L'étude des populations autochtones, captées successivement au cours des étés de 1974, 1975 et 1977 a été entreprise de mai 1977 à Janvier 1979. La croissance des huîtres redevient satisfaisantes ces dernières années; la qualité des mollusques s'améliore; les mortalités sont peut importantes. Le phénomène est plus sensible chez les populations les plus jeunes. Parallèlement nous notons une régression de la parasitose, tant au niveau des taux que des intensités de l'infestation.
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Maladie; Mortalité; Arcahon.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00015/12627/9515.pdf
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Localisation and characterization of acid phosphate activity in Bonamia ostreae (Ascetospora), an intrahemocytic protozoan parasite of the flat oyster Ostrea edulis (Bivalvia) ArchiMer
Hervio, Dominique; Chagot, Dominique; Godin, Pierre; Grizel, Henri; Mialhe, Eric.
Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the subtrate (cytidine 5' monophosphate). Lead phosphate precipiation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as "dense bodies". Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostrea is discussed.
Tipo: Text Palavras-chave: Acid phosphate activity; Ostrea edulis; Bonamia ostreae.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2755.pdf
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Maladie de la glande digestive de l'huître plate ArchiMer
Comps, Michel.
Tipo: Text Palavras-chave: Ostrea edulis; Etiologie; Prophylaxie et traitement; Histopathologie; Ostrea edulis; Etiology; Control; Histopathology.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/00140/25079/23187.pdf
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Maladie de la glande digestive de l'huître plate - Etude histochimique ArchiMer
Morel, Marc; Tige, Gilbert.
The histochemical study conducted here aims to bring to light the different biochemical components of the parasite. We carried out a series of analyses focusing on the main features known to this parasite: basic "primary" cell (stage 1), included "secondary" cells (stage 2), groups of "secondary" cells isolated by a membrane with or without "refracting clusters" (stage 4 and 3). The results of these histochemical tests are described in this work.
Tipo: Text Palavras-chave: Histochimie; Pathologie; Maladie; Ostrea edulis; Huître.
Ano: 1974 URL: http://archimer.ifremer.fr/doc/1974/publication-6287.PDF
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